Automated genotyping of microsatellite loci from feces with high throughput sequences.

ABSTRACT: Ecological and conservation genetic studies often use noninvasive sampling, especially with elusive or endangered species. Because microsatellites are generally short in length, they can be amplified from low quality samples such as feces. Microsatellites are highly polymorphic so few markers are enough for reliable individual identification, kinship determination, or population characterization. However, the genotyping process from feces is expensive and time consuming. Given next-generation sequencing (NGS) and recent software developments, automated microsatellite genotyping from NGS data may now be possible. These software packages infer the genotypes directly from sequence reads, increasing throughput. Here we evaluate the performance of four software packages to genotype microsatelli

SUBMITTER: Salado I 

PROVIDER: S-EPMC8544849 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature