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Species-specific PCR assay for the detection of Babesia odocoilei.

ABSTRACT: We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.

SUBMITTER: Burgess HJ

PROVIDER: S-EPMC8546463 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Species-specific PCR assay for the detection of Babesia odocoilei.

Burgess Hilary J HJ Lockerbie Betty P BP Ayalew Lisanework E LE Dibernardo Antonia A Hrazdilová Kristýna K Modry David D Bollinger Trent K TK

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 20210922 6

We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding pat ...[more]

PMID: 34550025

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