Mechanisms for Electron Uptake by Methanosarcina acetivorans during Direct Interspecies Electron Transfer.

ABSTRACT: Direct interspecies electron transfer (DIET) between bacteria and methanogenic archaea appears to be an important syntrophy in both natural and engineered methanogenic environments. However, the electrical connections on the outer surface of methanogens and the subsequent processing of electrons for carbon dioxide reduction to methane are poorly understood. Here, we report that the genetically tractable methanogen Methanosarcina acetivorans can grow via DIET in coculture with Geobacter metallireducens serving as the electron-donating partner. Comparison of gene expression patterns in M. acetivorans grown in coculture versus pure-culture growth on acetate revealed that transcripts for the outer-surface multiheme c-type cytochrome MmcA were higher during DIET-based growth. Dele

SUBMITTER: Holmes DE 

PROVIDER: S-EPMC8546582 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature