Proteome Regulation Patterns Determine Escherichia coli Wild-Type and Mutant Phenotypes.

ABSTRACT: It is generally recognized that proteins constitute the key cellular component in shaping microbial phenotypes. Due to limited cellular resources and space, optimal allocation of proteins is crucial for microbes to facilitate maximum proliferation rates while allowing a flexible response to environmental changes. To account for the growth condition-dependent proteome in the constraint-based metabolic modeling of Escherichia coli, we consolidated a coarse-grained protein allocation approach with the explicit consideration of enzymatic constraints on reaction fluxes. Besides representing physiologically relevant wild-type phenotypes and flux distributions, the resulting protein allocation model (PAM) advances the predictability of the metabolic responses to genetic perturbations. A main driv

SUBMITTER: Alter TB 

PROVIDER: S-EPMC8546978 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature