Diverse inflammatory threats modulate astrocytes Ca²⁺ signaling via connexin43 hemichannels in organotypic spinal slices.

ABSTRACT: Neuroinflammation is an escalation factor shared by a vast range of central nervous system (CNS) pathologies, from neurodegenerative diseases to neuropsychiatric disorders. CNS immune status emerges by the integration of the responses of resident and not resident cells, leading to alterations in neural circuits functions. To explore spinal cord astrocyte reactivity to inflammatory threats we focused our study on the effects of local inflammation in a controlled micro-environment, the organotypic spinal slices, developed from the spinal cord of mouse embryos. These organ cultures represent a complex in vitro model where sensory-motor cytoarchitecture, synaptic properties and spinal cord resident cells, are retained in a 3D fashion and we recently exploit these cultures to model two diverse

SUBMITTER: Panattoni G 

PROVIDER: S-EPMC8547100 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature