A Simple, Cost-Effective, and Automation-Friendly Direct PCR Approach for Bacterial Community Analysis.

ABSTRACT: Bacterial communities in water, soil, and humans play an essential role in environmental ecology and human health. PCR-based amplicon analysis, such as 16S rRNA sequencing, is a fundamental tool for quantifying and studying microbial composition, dynamics, and interactions. However, given the complexity of microbial communities, a substantial number of samples becomes necessary for analyses that parse the factors that determine microbial composition. A common bottleneck in performing these kinds of experiments is genomic DNA (gDNA) extraction, which is time-consuming, expensive, and often biased based on the types of species present. Direct PCR method is a potentially simpler and more accurate alternative to gDNA extraction methods that do not require the intervening purification step. In

SUBMITTER: Song F 

PROVIDER: S-EPMC8547444 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature