Streptococcus pyogenes Forms Serotype- and Local Environment-Dependent Interspecies Protein Complexes.

ABSTRACT: Streptococcus pyogenes is known to cause both mucosal and systemic infections in humans. In this study, we used a combination of quantitative and structural mass spectrometry techniques to determine the composition and structure of the interaction network formed between human plasma proteins and the surfaces of different S. pyogenes serotypes. Quantitative network analysis revealed that S. pyogenes forms serotype-specific interaction networks that are highly dependent on the domain arrangement of the surface-attached M protein. Subsequent structural mass spectrometry analysis and computational modeling of one of the M proteins, M28, revealed that the network structure changes across different host microenvironments. We report that M28 binds secretory IgA via two separate binding sites with

SUBMITTER: Chowdhury S 

PROVIDER: S-EPMC8547449 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature