Metagenomic Sequencing for Identification of Xylella fastidiosa from Leaf Samples.

ABSTRACT: Xylella fastidiosa (Xf) is a globally distributed plant-pathogenic bacterium. The primary control strategy for Xf diseases is eradicating infected plants; therefore, timely and accurate detection is necessary to prevent crop losses and further pathogen dispersal. Conventional Xf diagnostics primarily relies on quantitative PCR (qPCR) assays. However, these methods do not consider new or emerging variants due to pathogen genetic recombination and sensitivity limitations. We developed and tested a metagenomics pipeline using in-house short-read sequencing as a complementary approach for affordable, fast, and highly accurate Xf detection. We used metagenomics to identify Xf to the strain level in single- and mixed-infected plant samples at concentrations as

SUBMITTER: Roman-Reyna V 

PROVIDER: S-EPMC8547472 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature