Viral Inactivation Impacts Microbiome Estimates in a Tissue-Specific Manner.

ABSTRACT: The global emergence of novel pathogenic viruses presents an important challenge for research, as high biosafety levels are required to process samples. While inactivation of infectious agents facilitates the use of less stringent safety conditions, its effect on other biological entities of interest present in the sample is generally unknown. Here, we analyzed the effect of five inactivation methods (heat, ethanol, formaldehyde, psoralen, and TRIzol) on microbiome composition and diversity in samples collected from four different body sites (gut, nasal, oral, and skin) and compared them against untreated samples from the same tissues. We performed 16S rRNA gene sequencing and estimated abundance and diversity of bacterial taxa present in all samples. Nasal and skin samples were the most a

SUBMITTER: Boix-Amoros A 

PROVIDER: S-EPMC8547476 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature