The GH19 Engineering Database: Sequence diversity, substrate scope, and evolution in glycoside hydrolase family 19.

ABSTRACT: The glycoside hydrolase 19 (GH19) is a bifunctional family of chitinases and endolysins, which have been studied for the control of plant fungal pests, the recycle of chitin biomass, and the treatment of multi-drug resistant bacteria. The GH19 domain-containing sequences (22,461) were divided into a chitinase and an endolysin subfamily by analyzing sequence networks, guided by taxonomy and the substrate specificity of characterized enzymes. The chitinase subfamily was split into seventeen groups, thus extending the previous classification. The endolysin subfamily is more diverse and consists of thirty-four groups. Despite their sequence diversity, twenty-six residues are conserved in chitinases and endolysins, which can be distinguished by two specific sequence patterns at six and four pos

SUBMITTER: Orlando M 

PROVIDER: S-EPMC8547705 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature