Influence of the anatomical features of different brain regions on the spatial localization of fiber photometry signals.

ABSTRACT: Fiber photometry is widely used in neuroscience labs for in vivo detection of functional fluorescence from optical indicators of neuronal activity with a simple optical fiber. The fiber is commonly placed next to the region of interest to both excite and collect the fluorescence signal. However, the path of both excitation and fluorescence photons is altered by the uneven optical properties of the brain, due to local variation of the refractive index, different cellular types, densities and shapes. Nonetheless, the effect of the local anatomy on the actual shape and extent of the volume of tissue that interfaces with the fiber has received little attention so far. To fill this gap, we measured the size and shape of fiber photometry efficiency field in the primary motor and somatosen

SUBMITTER: Montinaro C 

PROVIDER: S-EPMC8547979 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature