Human retinal organoids release extracellular vesicles that regulate gene expression in target human retinal progenitor cells.

ABSTRACT: The mechanisms underlying retinal development have not been completely elucidated. Extracellular vesicles (EVs) are novel essential mediators of cell-to-cell communication with emerging roles in developmental processes. Nevertheless, the identification of EVs in human retinal tissue, characterization of their cargo, and analysis of their potential role in retina development has not been accomplished. Three-dimensional retinal tissue derived from human induced pluripotent stem cells (hiPSC) provide an ideal developmental system to achieve this goal. Here we report that hiPSC-derived retinal organoids release exosomes and microvesicles with small noncoding RNA cargo. EV miRNA cargo-predicted targetome correlates with Gene Ontology (GO) pathways involved in mechanisms of retinogenesis relevan

SUBMITTER: Zhou J 

PROVIDER: S-EPMC8548301 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature