Vesicle-Based Sensors for Extracellular Potassium Detection.

ABSTRACT:

Introduction

The design of sensors that can detect biological ions in situ remains challenging. While many fluorescent indicators exist that can provide a fast, easy readout, they are often nonspecific, particularly to ions with similar charge states. To address this issue, we developed a vesicle-based sensor that harnesses membrane channels to gate access of potassium (K⁺) ions to an encapsulated fluorescent indicator.

Methods

We assembled phospholipid vesicles that incorporated valinomycin, a K⁺ specific membrane transporter, and that encapsulated benzofuran isophthalate (PBFI), a K⁺ sensitive dye that nonspecifically fluoresces in the presence of other ions, like sodium (Na⁺). The specificity, kinetics, and reversibility

SUBMITTER: Boyd MA 

PROVIDER: S-EPMC8548450 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature