Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture.

ABSTRACT: Discovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates. The first structure in nascent centrioles is a cartwheel, which comprises stacked 9-fold symmetrical SAS-6 ring polymers emerging orthogonal to a surface surrounding each resident centriole. The mechanisms through which SAS-6 polymerization ensures centriole organelle architecture remain elusive. We deploy photothermally-actuated off-resonance tapping high-speed atomic force microscopy to decipher surface SAS-6 self-assembly mechanisms. We show that the surface shifts the reaction equilibrium by ~10⁴ compared to solution. Moreove

SUBMITTER: Banterle N 

PROVIDER: S-EPMC8548535 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature