Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm.

ABSTRACT: Red sea bream iridoviral disease (RSIVD) causes high economic damage in mariculture in Asian countries. However, there is little information on the source of infection and viral dynamics in fish farms. In the present study, the dynamics of RSIV in a fish farm that mainly reared juveniles and broodstocks of red sea bream (Pagrus major) were monitored over 3 years (2016 to 2018) by targeting environmental DNA (eDNA) of seawater. Our monitoring demonstrated that red sea bream iridovirus (RSIV) was detected from the eDNA at least 5 days before an RSIVD outbreak in the juveniles. The viral loads of eDNA during the outbreak were highly associated with the numbers for daily mortality, and they reached a peak of 10⁶ copies/liter seawater in late July in 2017, when daily mortality exceed

SUBMITTER: Kawato Y 

PROVIDER: S-EPMC8549737 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature