Intrabody-Induced Cell Death by Targeting the T. brucei Cytoskeletal Protein TbBILBO1.

ABSTRACT: Trypanosoma brucei belongs to a genus of protists that cause life-threatening and economically important diseases of human and animal populations in Sub-Saharan Africa. T. brucei cells are covered in surface glycoproteins, some of which are used to escape the host immune system. Exo-/endocytotic trafficking of these and other molecules occurs via a single copy organelle called the flagellar pocket (FP). The FP is maintained and enclosed around the flagellum by the flagellar pocket collar (FPC). To date, the most important cytoskeletal component of the FPC is an essential calcium-binding, polymer-forming protein called TbBILBO1. In searching for novel tools to study this protein, we raised nanobodies (Nb) against purified, full-length TbBILBO1. Nanobodies were selected accordi

SUBMITTER: Broster Reix CE 

PROVIDER: S-EPMC8549753 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature