Liposome fusion with orthogonal coiled coil peptides as fusogens: the efficacy of roleplaying peptides.

ABSTRACT: Biological membrane fusion is a highly specific and coordinated process as a multitude of vesicular fusion events proceed simultaneously in a complex environment with minimal off-target delivery. In this study, we develop a liposomal fusion model system with specific recognition using lipidated derivatives of a set of four de novo designed heterodimeric coiled coil (CC) peptide pairs. Content mixing was only obtained between liposomes functionalized with complementary peptides, demonstrating both fusogenic activity of CC peptides and the specificity of this model system. The diverse peptide fusogens revealed important relationships between the fusogenic efficacy and the peptide characteristics. The fusion efficiency increased from 20% to 70% as affinity between complementary peptide

SUBMITTER: Daudey GA 

PROVIDER: S-EPMC8549789 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature