Local delivery of FTY720 induces neutrophil activation through chemokine signaling in an oronasal fistula model.

ABSTRACT:

Purpose

Cleft palate repair surgeries lack a regenerative reconstructive option and, in many cases, develop complications including oronasal fistula (ONF). Our group has developed a novel murine phenocopy of ONF to study the oral cavity wound healing program. Using this model, our team previously identified that delivery of FTY720 on a nanofiber scaffold had a unique immunomodulatory effect directing macrophages and monocytes into a pro-regenerative state during ONF healing. Here, the objective of this study was to determine the effects of local biomaterial-based FTY720 delivery in the ONF model on the early bulk gene expression and neutrophil phenotypic response within the regenerating tissue.

Methods

Using a mouse model of ONF formation, a palate defect was created and was

SUBMITTER: Amanso AM 

PROVIDER: S-EPMC8549964 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature