Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF.

ABSTRACT:

Aims

We sought to develop a novel experimental system which enabled application of iodinated contrast media to in vivo plant roots intact in soil and was compatible with time-resolved synchrotron X-ray computed tomography imaging. The system was developed to overcome issues of low contrast to noise within X-ray computed tomography images of plant roots and soil environments, the latter of which can complicate image processing and result in the loss of anatomical information.

Methods

To demonstrate the efficacy of the system we employ the novel use of both synchrotron X-ray computed tomography and synchrotron X-ray fluorescence mapping to capture the translocation of the contrast media through root vasculature into the leaves.

Results

With the application of contrast m

SUBMITTER: Scotson CP 

PROVIDER: S-EPMC8550435 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature