CircFL-seq reveals full-length circular RNAs with rolling circular reverse transcription and nanopore sequencing.

ABSTRACT: Circular RNAs (circRNAs) act through multiple mechanisms via their sequence features to fine-tune gene expression networks. Due to overlapping sequences with linear cognates, identifying internal sequences of circRNAs remains a challenge, which hinders a comprehensive understanding of circRNA functions and mechanisms. Here, based on rolling circular reverse transcription and nanopore sequencing, we developed circFL-seq, a full-length circRNA sequencing method, to profile circRNA at the isoform level. With a customized computational pipeline to directly identify full-length sequences from rolling circular reads, we reconstructed 77,606 high-quality circRNAs from seven human cell lines and two human tissues. circFL-seq benefits from rolling circles and long-read sequencing, and the results s

SUBMITTER: Liu Z 

PROVIDER: S-EPMC8550772 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature