Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle.

ABSTRACT: While the importance of RNA localization in highly differentiated cells is well appreciated, basic principles of RNA localization in skeletal muscle remain poorly characterized. Here, we develop a method to detect and quantify single molecule RNA localization patterns in skeletal myofibers, and uncover a critical role for directed transport of RNPs in muscle. We find that RNAs localize and are translated along sarcomere Z-disks, dispersing tens of microns from progenitor nuclei, regardless of encoded protein function. We find that directed transport along the lattice-like microtubule network of myofibers becomes essential to achieve this localization pattern as muscle development progresses; disruption of this network leads to extreme accumulation of RNPs and nascent protein around myonucl

SUBMITTER: Denes LT 

PROVIDER: S-EPMC8551216 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature