Establishment of novel in vitro culture system with the ability to reproduce oral biofilm formation on dental materials.

ABSTRACT: Intensive research has been conducted with the aim of developing dental restorative/prosthetic materials with antibacterial and anti-biofilm effects that contribute to controlling bacterial infection in the oral cavity. In situ evaluations were performed to assess the clinical efficacy of these materials by exposing them to oral environments. However, it is difficult to recruit many participants to collect sufficient amount of data for scientific analysis. This study aimed to assemble an original flow-cell type bioreactor equipped with two flow routes and assess its usefulness by evaluating the ability to reproduce in situ oral biofilms formed on restorative materials. A drop of bacterial suspension collected from human saliva and 0.2% sucrose solution was introduced into the assembled bio

SUBMITTER: Kohno T 

PROVIDER: S-EPMC8551238 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature