Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes.
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ABSTRACT:
SUBMITTER: Zhang JZ
PROVIDER: S-EPMC8551496 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
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Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes.
Zhang Joe Z JZ Zhao Shane Rui SR Tu Chengyi C Pang Paul P Zhang Mao M Wu Joseph C JC
STAR protocols 20211008 4
Multiple strategies have been developed to efficiently differentiate human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Here, we describe a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium (Ca<sup>2+</sup>) handling, and action potential. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021). ...[more]
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