Diversity of cell death signaling pathways in macrophages upon infection with modified vaccinia virus Ankara (MVA).

ABSTRACT: Regulated cell death frequently occurs upon infection by intracellular pathogens, and extent and regulation is often cell-type-specific. We aimed to identify the cell death-signaling pathways triggered in macrophages by infection with modified vaccinia virus Ankara (MVA), an attenuated strain of vaccinia virus used in vaccination. While most target cells seem to be protected by antiapoptotic proteins encoded in the MVA genome, macrophages die when infected with MVA. We targeted key signaling components of specific cell death-pathways and pattern recognition-pathways using genome editing and small molecule inhibitors in an in vitro murine macrophage differentiation model. Upon infection with MVA, we observed activation of mitochondrial and death-receptor-induced apoptosis-pathways as well a

SUBMITTER: Klaas L 

PROVIDER: S-EPMC8551665 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature