Three-dimensional organoid culture unveils resistance to clinical therapies in adult and pediatric glioblastoma.

ABSTRACT:

Background

Glioblastoma (GBM) is the most common primary brain tumor with a dismal prognosis. The inherent cellular diversity and interactions within tumor microenvironments represent significant challenges to effective treatment. Traditional culture methods such as adherent or sphere cultures may mask such complexities whereas three-dimensional (3D) organoid culture systems derived from patient cancer stem cells (CSCs) can preserve cellular complexity and microenvironments. The objective of this study was to determine if GBM organoids may offer a platform, complimentary to traditional sphere culture methods, to recapitulate patterns of clinical drug resistance arising from 3D growth.

Methods

Adult and pediatric surgical specimens were collected and established as organoids.

SUBMITTER: Sundar SJ 

PROVIDER: S-EPMC8551697 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature