Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

ABSTRACT:

Background & aims

Tissue-resident memory T (Trm) cells, both of the CD4 and CD8 lineage, have been implicated in disease flares in inflammatory bowel disease. However, data are conflicting regarding the profile of human CD8⁺ Trm cells, with studies suggesting both proinflammatory and regulatory functions. It is crucial to understand the functional profile of these cells in the context of (new) therapeutic strategies targeting (trafficking of) gut Trm cells.

Methods

Here, we performed imaging mass cytometry, flow cytometry, and RNA-sequencing to compare lamina propria and intraepithelial CD103^+/-CD69⁺CD8⁺ Trm cells in healthy control subjects and patients with active ileal Crohn's disease.

Results

Our data revealed that lamin

SUBMITTER: Lutter L 

PROVIDER: S-EPMC8551698 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature