A Novel Microphysiological Colon Platform to Decipher Mechanisms Driving Human Intestinal Permeability.

ABSTRACT:

Background & aims

The limited availability of organoid systems that mimic the molecular signatures and architecture of human intestinal epithelium has been an impediment to allowing them to be harnessed for the development of therapeutics as well as physiological insights. We developed a microphysiological Organ-on-Chip (Emulate, Inc, Boston, MA) platform designed to mimic properties of human intestinal epithelium leading to insights into barrier integrity.

Methods

We combined the human biopsy-derived leucine-rich repeat-containing G-protein-coupled receptor 5-positive organoids and Organ-on-Chip technologies to establish a micro-engineered human Colon Intestine-Chip (Emulate, Inc, Boston, MA). We characterized the proximity of the model to human tissue and organoids maintai

SUBMITTER: Apostolou A 

PROVIDER: S-EPMC8551844 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature