Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways.
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ABSTRACT:
SUBMITTER: Dobosh B
PROVIDER: S-EPMC8551927 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways.
Dobosh Brian B Giacalone Vincent D VD Margaroli Camilla C Tirouvanziam Rabindra R
STAR protocols 20211021 4
Neutrophils are difficult to study, particularly in tissues, due to their short half-life and propensity for activation. We describe an organotypic airway model that uses patient airway fluid to enable the transmigration of blood neutrophils to acquire an airway-like phenotype in order to better understand their contribution to airway diseases. In particular, we showcase how conditioned neutrophils modulate their bacteria-killing abilities. For complete details on the use and execution of this p ...[more]
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