Development and Validation of a Novel LC-MS/MS Assay for C-Peptide in Human Serum.

ABSTRACT:

Introduction

C-peptide is used as a marker of endogenous insulin secretion in the assessment of residual β-cell function in diabetes and in the diagnostic workup of hypoglycemia. Previously developed LC-MS/MS methods to quantify serum concentrations of C-peptide have monitored intact peptide, which ionizes poorly. As a result, methods have leveraged immunoaffinity enrichment or two-dimensional chromatography. In this study, we aimed to use proteolysis during sample preparation to enhance the sensitivity of traditional LC-MS/MS.

Methods

Due to the absence of arginine and lysine residues in C-peptide, we utilized Glu-C as the proteolytic enzyme in the method. After protein precipitation using acetonitrile and solid phase extraction with mixed anion exchange, lower molecular we

SUBMITTER: Owusu BY 

PROVIDER: S-EPMC8553002 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature