Phosphoproteomic profiling of the hippocampus of offspring rats exposed to prenatal stress.

ABSTRACT:

Introduction

Prenatal stress (PS) can cause depression in offspring. However, the underlying biological mechanism of these influences is still unclear. This work was implemented to investigate the molecular mechanisms of depressive-like behavior of offspring rats insulted with PS.

Methods

Relative quantitative phosphoproteomics of the hippocampus of PS susceptibility (PS-S) and control (CON) rat offspring was performed using  liquid chromatography-tandem mass spectrometry to confirm known pathways and to identify new mechanisms involved in depression.

Results

A total of 6790 phosphopeptides, 9817 phosphorylation sites, and 2978 phosphoproteins were detected. Among the 2978 phosphoproteins, 1760 (59.09%) had more than two phosphorylated sites, the ENSRNOP00000023460 pr

SUBMITTER: Li Q 

PROVIDER: S-EPMC8553319 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature