Small Protein Enrichment Improves Proteomics Detection of sORF Encoded Polypeptides.

ABSTRACT: With the rapid growth in the number of sequenced genomes, genome annotation efforts became almost exclusively reliant on automated pipelines. Despite their unquestionable utility, these methods have been shown to underestimate the true complexity of the studied genomes, with small open reading frames (sORFs; ORFs typically considered shorter than 300 nucleotides) and, in consequence, their protein products (sORF encoded polypeptides or SEPs) being the primary example of a poorly annotated and highly underexplored class of genomic elements. With the advent of advanced translatomics such as ribosome profiling, reannotation efforts have progressed a great deal in providing translation evidence for numerous, previously unannotated sORFs. However, proteomics validation of these riboproteogenomi

SUBMITTER: Fijalkowski I 

PROVIDER: S-EPMC8554064 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature