Mapping Pulmonary and Systemic Inflammation in Preschool Aged Children With Cystic Fibrosis.

ABSTRACT: The immune landscape of the paediatric respiratory system remains largely uncharacterised and as a result, the mechanisms of globally important childhood respiratory diseases remain poorly understood. In this work, we used high parameter flow cytometry and inflammatory cytokine profiling to map the local [bronchoalveolar lavage (BAL)] and systemic (whole blood) immune response in preschool aged children with cystic fibrosis (CF) and aged-matched healthy controls. We demonstrate that children with CF show pulmonary infiltration of CD66b⁺ granulocytes and increased levels of MIP-1α, MIG, MCP-1, IL-8, and IL-6 in BAL relative to healthy control children. Proportions of systemic neutrophils positively correlated with age in children with CF, whilst systemic CD4 T cells and B cells w

SUBMITTER: Shanthikumar S 

PROVIDER: S-EPMC8554310 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature