Metagenomic next-generation sequencing to identify pathogens and cancer in lung biopsy tissue.

ABSTRACT:

Background

Lung biopsy tissue samples can be used for infection detection and cancer diagnosis. Metagenomic next-generation sequencing (mNGS) has the potential to further improve diagnosis.

Methods

From July 2018 to May 2020, lung biopsy samples of 133 patients with suspected pulmonary infection or abnormal imaging findings were collected and subjected to clinical microbiological testing, Illumina and Nanopore sequencing to identify pathogens. The neural networks were pretrained by extracting features of human reads from 2,095 metagenomic next-generation sequencing results, and the human reads of lung biopsy samples were entered into the validated pipeline to predict the risk of cancer.

Findings

Based on the pathogen-cancer detection pipeline, the Illumina platform sh

SUBMITTER: Guo Y 

PROVIDER: S-EPMC8554462 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature