In Vivo Measurement of Granzyme Proteolysis from Activated Immune Cells with PET.

ABSTRACT: The biology of human granzymes remains enigmatic in part due to our inability to probe their functions outside of in vitro assays or animal models with divergent granzyme species. We hypothesize that the biology of human granzymes could be better elaborated with a translational imaging technology to reveal the contexts in which granzymes are secreted and biochemically active in vivo. Here, we advance toward this goal by engineering a Granzyme targeting Restricted Interaction Peptide specific to family member B (GRIP B) to measure secreted granzyme B (GZMB) biochemistry with positron emission tomography. A proteolytic cleavage of ⁶⁴Cu-labeled GRIP B liberates a radiolabeled form of Temporin L, which sequesters the radioisotope by binding to adjacent phospholipid bilayers. Thus, a

SUBMITTER: Zhao N 

PROVIDER: S-EPMC8554823 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature