Dataset Information

Serum peptidome: diagnostic window into pathogenic processes following occupational exposure to carbon nanomaterials.

ABSTRACT:

Background

Growing industrial use of carbon nanotubes and nanofibers (CNT/F) warrants consideration of human health outcomes. CNT/F produces pulmonary, cardiovascular, and other toxic effects in animals along with a significant release of bioactive peptides into the circulation, the augmented serum peptidome. While epidemiology among CNT/F workers reports on few acute symptoms, there remains concern over sub-clinical CNT/F effects that may prime for chronic disease, necessitating sensitive health outcome diagnostic markers for longitudinal follow-up.

Methods

Here, the serum peptidome was assessed for its biomarker potential in detecting sub-symptomatic pathobiology among CNT/F workers using label-free data-independent mass spectrometry. Studies employed a stratified design between High (> 0.5 µg/m³) and Low (< 0.1 µg/m³) inhalable CNT/F exposures in the industrial setting. Peptide biomarker model building and refinement employed linear regression and partial least squared discriminant analyses. Top-ranked peptides were then sequence identified and evaluated for pathological-relevance.

Results

In total, 41 peptides were found to be highly discriminatory after model building with a strong linear correlation to personal CNT/F exposure. The top-five peptide model offered ideal prediction with high accuracy (Q² = 0.99916). Unsupervised validation affirmed 43.5% of the serum peptidomic variance was attributable to CNT/F exposure. Peptide sequence identification reveals a predominant association with vascular pathology. ARHGAP21, ADAM15 and PLPP3 peptides suggest heightened cardiovasculature permeability and F13A1, FBN1 and VWDE peptides infer a pro-thrombotic state among High CNT/F workers.

Conclusions

The serum peptidome affords a diagnostic window into sub-symptomatic pathology among CNT/F exposed workers for longitudinal monitoring of systemic health risks.

SUBMITTER: Mostovenko E

PROVIDER: S-EPMC8555107 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Publications

Serum peptidome: diagnostic window into pathogenic processes following occupational exposure to carbon nanomaterials.

Mostovenko Ekaterina E Dahm Matthew M MM Schubauer-Berigan Mary K MK Eye Tracy T Erdely Aaron A Young Tamara L TL Campen Matthew J MJ Ottens Andrew K AK

Particle and fibre toxicology 20211028 1

<h4>Background</h4>Growing industrial use of carbon nanotubes and nanofibers (CNT/F) warrants consideration of human health outcomes. CNT/F produces pulmonary, cardiovascular, and other toxic effects in animals along with a significant release of bioactive peptides into the circulation, the augmented serum peptidome. While epidemiology among CNT/F workers reports on few acute symptoms, there remains concern over sub-clinical CNT/F effects that may prime for chronic disease, necessitating sensiti ...[more]

PMID: 34711247

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Dataset Information

Serum peptidome: diagnostic window into pathogenic processes following occupational exposure to carbon nanomaterials.

Background

Methods

Results

Conclusions

Publications

Serum peptidome: diagnostic window into pathogenic processes following occupational exposure to carbon nanomaterials.

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