Red blood cells differentiated in vitro using sequential liquid and semi-solid culture as a pre-clinical model.

ABSTRACT: The in vitro erythrocyte differentiation model remains a strong, clinically relevant tool to model erythroid development in normal and disease related hematopoiesis. This model also has application to developing therapeutics for diseases related to red blood cells such as sickle cell anemia where targeting increased expression of fetal hemoglobin has been a major emphasis. Since the original protocol's publication in 2002, many groups have published modified methodologies to address issues in efficiency of maturation and terminal enucleation, as well as in scalability. While all reports have merit and show efficient enucleation, the methodologies used vary widely in technique and cytokine content. Additionally, despite the strengths in these methods, reproducibility of efficient differenti

SUBMITTER: Cannon M 

PROVIDER: S-EPMC8555309 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature