An intrinsically disordered nascent protein interacts with specific regions of the ribosomal surface near the exit tunnel.

ABSTRACT: The influence of the ribosome on nascent chains is poorly understood, especially in the case of proteins devoid of signal or arrest sequences. Here, we provide explicit evidence for the interaction of specific ribosomal proteins with ribosome-bound nascent chains (RNCs). We target RNCs pertaining to the intrinsically disordered protein PIR and a number of mutants bearing a variable net charge. All the constructs analyzed in this work lack N-terminal signal sequences. By a combination chemical crosslinking and Western-blotting, we find that all RNCs interact with ribosomal protein L23 and that longer nascent chains also weakly interact with L29. The interacting proteins are spatially clustered on a specific region of the large ribosomal subunit, close to the exit tunnel. Based on chain-leng

SUBMITTER: Guzman-Luna V 

PROVIDER: S-EPMC8556260 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature