Transcriptomic analysis of Rhodococcus opacus R7 grown on polyethylene by RNA-seq.

ABSTRACT: Plastic waste management has become a global issue. Polyethylene (PE) is the most abundant synthetic plastic worldwide, and one of the most resistant to biodegradation. Indeed, few bacteria can degrade polyethylene. In this paper, the transcriptomic analysis unveiled for the first time Rhodococcus opacus R7 complex genetic system based on diverse oxidoreductases for polyethylene biodegradation. The RNA-seq allowed uncovering genes putatively involved in the first step of oxidation. In-depth investigations through preliminary bioinformatic analyses and enzymatic assays on the supernatant of R7 grown in the presence of PE confirmed the activation of genes encoding laccase-like enzymes. Moreover, the transcriptomic data allowed identifying candidate genes for the further steps of short alipha

SUBMITTER: Zampolli J 

PROVIDER: S-EPMC8556283 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature