Genetic polymorphisms of Vascular Endothelial Growth Factor (VEGF) associated with endometriosis in Nigerian women.

ABSTRACT:

Objective

To determine if genetic polymorphism of VEGF is associated with the development of endometriosis in Nigerian women.

Study design

Case control study of 100 women (50 healthy controls and 50 with endometriosis). Serum VEGF concentration of participants were determined using enzyme-linked immunosorbent assay (ELISA) technique. Genomic DNAs were isolated from peripheral blood samples and quantified by nanodrop spectrophotometer one. Single nucleotide polymorphisms genotyping was carried out by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).

Results

Mean age of participants was 32.96 ± 6.91 years for control and 32.04 ± 7.56 years for cases. VEGF levels in case and control groups were not statistically different (82.68 pg/ml [69

SUBMITTER: Babah OA 

PROVIDER: S-EPMC8556990 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature