Mistranslation Reduces Mutation Load in Evolving Proteins through Negative Epistasis with DNA Mutations.

ABSTRACT: Translational errors during protein synthesis cause phenotypic mutations that are several orders of magnitude more frequent than DNA mutations. Such phenotypic mutations may affect adaptive evolution through their interactions with DNA mutations. To study how mistranslation may affect the adaptive evolution of evolving proteins, we evolved populations of green fluorescent protein (GFP) in either high-mistranslation or low-mistranslation Escherichia coli hosts. In both hosts, we first evolved GFP under purifying selection for the ancestral phenotype green fluorescence, and then under directional selection toward the new phenotype yellow fluorescence. High-mistranslation populations evolved modestly higher yellow fluorescence during each generation of evolution than low-mistranslation popula

SUBMITTER: Zheng J 

PROVIDER: S-EPMC8557407 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature