Limited Plasmodium sporozoite gliding motility in the absence of TRAP family adhesins.

ABSTRACT:

Background

Plasmodium sporozoites are the highly motile forms of malaria-causing parasites that are transmitted by the mosquito to the vertebrate host. Sporozoites need to enter and cross several cellular and tissue barriers for which they employ a set of surface proteins. Three of these proteins are members of the thrombospondin related anonymous protein (TRAP) family. Here, potential additive, synergistic or antagonistic roles of these adhesion proteins were investigated.

Methods

Four transgenic Plasmodium berghei parasite lines that lacked two or all three of the TRAP family adhesins TRAP, TLP and TREP were generated using positive-negative selection. The parasite lines were investigated for their capacity to attach to and move on glass, their ability to egress from oocys

SUBMITTER: Beyer K 

PROVIDER: S-EPMC8557484 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature