Maternal methionine supplementation during gestation alters alternative splicing and DNA methylation in bovine skeletal muscle.

ABSTRACT:

Background

The evaluation of alternative splicing, including differential isoform expression and differential exon usage, can provide some insights on the transcriptional changes that occur in response to environmental perturbations. Maternal nutrition is considered a major intrauterine regulator of fetal developmental programming. The objective of this study was to assess potential changes in splicing events in the longissimus dorsi muscle of beef calves gestated under control or methionine-rich diets. RNA sequencing and whole-genome bisulfite sequencing were used to evaluate muscle transcriptome and methylome, respectively.

Results

Alternative splicing patterns were significantly altered by maternal methionine supplementation. Most of the altered genes were directly implic

SUBMITTER: Liu L 

PROVIDER: S-EPMC8557564 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature