Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy.

ABSTRACT: Background: Membrane potential (V _mem) exerts physiological influence across a wide range of time and space scales. To study V _mem in these diverse contexts, it is essential to accurately record absolute values of V _mem, rather than solely relative measurements. Materials and Methods: We use fluorescence lifetime imaging of a small molecule voltage sensitive dye (VF2.1.Cl) to estimate mV values of absolute membrane potential. Results: We test the consistency of VF2.1.Cl lifetime measurements performed on different single-photon counting instruments and find that they are in striking agreement (differences of <0.5 ps/mV in the slope and <50 ps in the y-intercept). We also demonstrate that

SUBMITTER: Lazzari-Dean JR 

PROVIDER: S-EPMC8558063 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature