Quality control in scRNA-Seq can discriminate pacemaker cells: the mtRNA bias.

ABSTRACT: Single-cell RNA-sequencing (scRNA-seq) provides high-resolution insights into complex tissues. Cardiac tissue, however, poses a major challenge due to the delicate isolation process and the large size of mature cardiomyocytes. Regardless of the experimental technique, captured cells are often impaired and some capture sites may contain multiple or no cells at all. All this refers to "low quality" potentially leading to data misinterpretation. Common standard quality control parameters involve the number of detected genes, transcripts per cell, and the fraction of transcripts from mitochondrial genes. While cutoffs for transcripts and genes per cell are usually user-defined for each experiment or individually calculated, a fixed threshold of 5% mitochondrial transcripts is standard and ofte

SUBMITTER: Galow AM 

PROVIDER: S-EPMC8558157 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature