Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants.

ABSTRACT: Green fluorescent protein (GFP) has been widely used for monitoring gene expression and protein localization in diverse organisms. However, highly sensitive imaging equipment, like fluorescence microscope, is usually required for the visualization of GFP, limitings its application to fixed locations in samples. A reporter that can be visualized in real-time regardless the shape, size and location of the target samples will increase the flexibility and efficiency of research work. Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species (Arabidopsis and tobacco) and two woody plant species (poplar and citrus). We observed bright fluorescence under UV light in all of the four plant species w

SUBMITTER: Yuan G 

PROVIDER: S-EPMC8558336 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature