Sequential in cis mutagenesis in vivo reveals various functions for CTCF sites at the mouse HoxD cluster.

ABSTRACT: Mammalian Hox gene clusters contain a range of CTCF binding sites. In addition to their importance in organizing a TAD border, which isolates the most posterior genes from the rest of the cluster, the positions and orientations of these sites suggest that CTCF may be instrumental in the selection of various subsets of contiguous genes, which are targets of distinct remote enhancers located in the flanking regulatory landscapes. We examined this possibility by producing an allelic series of cumulative in cis mutations in these sites, up to the abrogation of CTCF binding in the five sites located on one side of the TAD border. In the most impactful alleles, the global chromatin architecture of the locus was modified, yet not drastically, illustrating that CTCF sites located on

SUBMITTER: Amandio AR 

PROVIDER: S-EPMC8559674 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature