A systematic analysis of <i>Trypanosoma brucei</i> chromatin factors identifies novel protein interaction networks associated with sites of transcription initiation and termination.

ABSTRACT: Nucleosomes composed of histones are the fundamental units around which DNA is wrapped to form chromatin. Transcriptionally active euchromatin or repressive heterochromatin is regulated in part by the addition or removal of histone post-translational modifications (PTMs) by "writer" and "eraser" enzymes, respectively. Nucleosomal PTMs are recognized by a variety of "reader" proteins that alter gene expression accordingly. The histone tails of the evolutionarily divergent eukaryotic parasite Trypanosoma brucei have atypical sequences and PTMs distinct from those often considered universally conserved. Here we identify 65 predicted readers, writers, and erasers of histone acetylation and methylation encoded in the T. brucei genome and, by epitope tagging, systemically localize

SUBMITTER: Staneva DP 

PROVIDER: S-EPMC8559703 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature