MET1-Dependent DNA Methylation Represses Light Signaling and Influences Plant Regeneration in Arabidopsis.

ABSTRACT: Plant somatic cells can be reprogrammed into a pluripotent cell mass, called callus, which can be subsequently used for de novo shoot regeneration through a two-step in vitro tissue culture method. MET1-dependent CG methylation has been implicated in plant regeneration in Arabidopsis, because the met1-3 mutant exhibits increased shoot regeneration compared with the wild-type. To understand the role of MET1 in de novo shoot regeneration, we compared the genome-wide DNA methylomes and transcriptomes of wild-type and met1-3 callus and leaf. The CG methylation patterns were largely unchanged during leaf-to-callus transition, suggesting that the altered regeneration phenotype of met1-3 was caused by the constitutively hypomethylated genes, indepe

SUBMITTER: Shim S 

PROVIDER: S-EPMC8560584 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature