Mitochondrial Calcium uniporters are essential for meiotic progression in mouse oocytes by controlling Ca²⁺ entry.

ABSTRACT:

Objectives

The alteration of bioenergetics by oocytes in response to the demands of various biological processes plays a critical role in maintaining normal cellular physiology. However, little is known about the association between energy sensing and energy production with energy-dependent cellular processes like meiosis.

Materials and methods

We demonstrated that cell cycle-dependent mitochondrial Ca²⁺ connects energy sensing to mitochondrial activity in meiosis progression within mouse oocytes. Further, we established a model in mouse oocytes using siRNA knockdowns that target mitochondrial calcium uniporters (MCUs) in order to inhibit mitochondrial Ca²⁺ concentrations.

Results

Decreased numbers of oocytes successfully progressed to the germin

SUBMITTER: Zhang LY 

PROVIDER: S-EPMC8560604 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature