Arid1a regulates neural stem/progenitor cell proliferation and differentiation during cortical development.

ABSTRACT:

Objective

Neurodevelopmental diseases are common disorders caused by the disruption of essential neurodevelopmental processes. Recent human exome sequencing and genome-wide association studies have shown that mutations in the subunits of the SWI/SNF (BAF) complex are risk factors for neurodevelopmental diseases. Clinical studies have found that ARID1A (BAF250a) is the most frequently mutated SWI/SNF gene and its mutations lead to mental retardation and microcephaly. However, the function of ARID1A in brain development and its underlying mechanisms still remain elusive.

Methods

The present study used Cre/loxP system to generate an Arid1a conditional knockout mouse line. Cell proliferation, cell apoptosis and cell differentiation of NSPCs were studied by immunofluorescence sta

SUBMITTER: Liu X 

PROVIDER: S-EPMC8560606 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature